STREPTOMYCES FRADIAE, A POTENT PRODUCER OF CHOLESTEROL OXIDASE
نویسندگان: ثبت نشده
چکیده مقاله:
Thirteen species of Streptomycetes, Arthrobacters and Mycobacterium were studied for the determination of a potential cholesterol oxidase producer. Only Streptomyces fradiae and Streptomyces rishiriences were found to be capable of producing this enzyme. Streptomyces fradiae was selected for the production of cholesterol oxidase and cultured in different media to find optimum media and conditions for enzyme production. The optimum media was found (g/Lit): glucose, 12; starch, 9; yeast extract, 6 ;peptone, 4; (NH ) SO ,7.5; Tween 80,0.5 with cholesterol, 2 as an enzyme inducer. 3 4 ?C w as the optlmum temperature for the production of cholesterol oxidase
منابع مشابه
The tylosin producer, Streptomyces fradiae, contains a second valine dehydrogenase.
A second NAD-dependent valine dehydrogenase (VDH) of Streptomyces fradiae was detected and purified to homogeneity by affinity chromatography on Reactive-Blue 2 Sepharose followed by gel filtration and Mono Q fast protein liquid chromatography. The relative molecular masses of the native enzyme and its subunits were determined to be 80,000 and 41,000, respectively, indicating that the enzyme is...
متن کاملThe mycarose-biosynthetic genes of Streptomyces fradiae, producer of tylosin.
The tylCK region of the Streptomyces fradiae genome was sequenced, revealing an incomplete set of five tylC genes encoding all-but-one of the enzymes involved in the biosynthesis of mycarose. The latter is a 6-deoxyhexose sugar required during production of the macrolide antibiotic, tylosin. The missing mycarose-biosynthetic gene, tylCVI, was found about 50 kb distant from its functional partne...
متن کاملOnion, a Potent Inhibitor of Xantine Oxidase
Onion (Allium Cepa) contains high levels of flavonoids. Although there are many studies indicating the inhibitory effects of flavonoids on xanthine oxidase, there is no report on the effect of onion on this enzyme. Therefore, in the present study, the inhibitory effects of onion on xanthine oxidase are investigated. Fresh filtered juice of onion was prepared and its inhibitory effect on guine...
متن کاملOnion, a Potent Inhibitor of Xantine Oxidase
Onion (Allium Cepa) contains high levels of flavonoids. Although there are many studies indicating the inhibitory effects of flavonoids on xanthine oxidase, there is no report on the effect of onion on this enzyme. Therefore, in the present study, the inhibitory effects of onion on xanthine oxidase are investigated. Fresh filtered juice of onion was prepared and its inhibitory effect on guine...
متن کاملAmplified DNA in Streptomyces fradiae.
A spontaneous mutant of Streptomyces fradiae contained an amplifiable unit of DNA with a sequence length of approximately 10.5 kilobases that was amplified to approximately 500 copies per chromosome. The amplified DNA appears to be cryptic. SalI fragments of the amplified DNA were cloned into Escherichia coli to construct a restriction map and characterize the amplified DNA. The amplified DNA c...
متن کاملThe Production of Neomycin by Streptomyces Fradiae
Of basic importance to the study of antibiotic production by microorganisms is a chemically defined medium in which the effect of various nutrients, both organic and inorganic, can be studied with a minimum of interference from ill-defined substances. Such a medium is reported here for the production of neomycin by Streptomyces fradiae, together with studies on the influence of various nitrogen...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 10 شماره 3
صفحات -
تاریخ انتشار 1999-09-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023